Write a seqspec file for your custom datasets

Introduction to seqspec

Precellar relies on a seqspec file to understand the structure of your datasets. For details about seqspec, please refer to the seqspec documentation.

We have provided some seqspec templates for common datasets in the seqspec/templates directory. If you have a dataset that is not yet supported, you can check whether it is provided here.

Notes on writing seqspec files

Format

The seqspec file is a yaml file that adheres to the seqspec schema.

Customizing seqspec files

Sometimes the library structure varies from the default templates. For example, barcodes length and location can be different in different Cel-seq2 datasets. In this case, you can customize the seqspec file to match your dataset.